Qiagen plasmic midikit 121435/18/2023 ![]() The high-copy plasmids listed here contain mutated versions of this origin. * The pMB1 origin of replication is closely related to that of ColE1 and falls in the same incompatibility group. Origins of replication and copy numbers of various plasmids and cosmids A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture. A way to determine experimentally if the copy number of your plasmid is high or low is to perform a miniprep. This table can also be found online at the QIAGEN Plasmid Resource Center in the section ' Growth of bacterial cultures Plasmid Copy Number'. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.įind out which origin of replication your plasmid contains, and look at the table below for classification into high-copy or low-copy types. Prepacked QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. This protocol is designed for preparation of up to 20 g of high-copy plasmid or cosmid DNA using the QIAGEN Plasmid Mini Kit. The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. QIAfilter Maxi and Midi Cartridges use a syringe format and lysates are cleared rapidly by pushing the liquid through the filter. Knowledgeable and professional Product & Technical Support. 24/7 automatic processing of online orders. These products are not intended for the diagnosis, prevention, or treatment of a disease. Time/Run, Anion-exchange Technology, 1 Sample/Run, For Purification of up to 100g Transfection-grade Plasmid or. QIAfilter Plasmid Kits are intended for molecular biology applications. purified plasmid DNA, genomic DNA, or RNA from a variety of sample types. Kit contents: Qiagen Plasmid Midi Kit, 25 preps, 25 to 100mL Culture Volume, Manual (Centrifugation) Processing, 150 min. acid purification products to Qiagen nucleic acid purification products. Visit the QIAGEN plasmid kits web page to learn more. For purification of up to 10 mg transfection-grade plasmid or cosmid DNA. (Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium. Up to 100 g high-copy plasmid DNA is purified from 25100 ml culture. 12181) and the QIAGEN Plasmid Giga Kit (cat. For 250 high-purity plasmid minipreps: 250 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2. Classic QIAGEN Plasmid Midi Kits use gravity-flow QIAGEN-tip 100 anion-exchange tips for efficient purification of plasmid DNA. 19781) with the QIAGEN Plasmid Mega Kit (cat. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation, reducing plasmid-purification time by up to 1 hour.Īs an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation, you can use the QIAfilter Mega-Giga Cartridges (cat. 12143 QIAGEN Plasmid Midi Kit (25) 12162 QIAGEN Plasmid Maxi Kit (10) 12163 QIAGEN Plasmid Maxi Kit (25) 12262 QIAfilter Plasmid Maxi Kit (10) 12362 EndoFree Plasmid Maxi Kit (10) PCR & RT-PCR Reagents 210210 QIAGEN One Step PCR Kit 203603 HotStarTaq Plus DNA Polym(250u) 203605 HotStarTaqPlusDNA Polym(1000u) 201203 Taq DNA Polymerase (250u. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).QIAfilter Cartridges, provided in QIAfilter, HiSpeed, and EndoFree Plasmid Kits, are special filter units designed to replace centrifugation following alkaline lysis of bacterial cells. ![]() QIAwell Plasmid Purification System: ultrapure plasmid minipreps. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. The use of a vacuum manifold allows purification of up to 24 samples in parallel, reducing the need for hands-on time. Only negative point is the results are inconsistent. QIAGEN Pty Ltd PO Box 25 Clifton Hill Victoria 3068. The QIAGEN Plasmid Plus Midi Kit enables ultrafast, large-scale purification of up to 250 g of highly pure plasmid DNA. 3 It can process 20-30 samples same-time, at high efficiency. QIAGEN Plasmid Kits are developed, designed and sold for research purposes only. It can obtain high quality plasmid DNA, easy to use for transfection. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. Application Area: Endotoxin-Free Plasmid DNA Preparation. QIAGEN Plasmid Mini Kit (QIAGEN, catalog number: 12123) QIAGEN Plasmid Midi Kit (QIAGEN, catalog number: 12143) QIAquick Gel Extraction Kit (QIAGEN. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function.
0 Comments
Leave a Reply. |